Submit Manuscript  

Article Details


Development of Sertaconazole Nitrate Loaded Nanostructured Lipid Carriers Gel Using Central Composite Design: In-vitro and Ex-vivo Evaluation

Author(s):

Moreshwar Patil*, Pallavi Bhagade, Meghana Amale, Sandeep Sonawane and Sanjay Kshirsagar   Pages 1 - 12 ( 12 )

Abstract:


Aim: The aim of this study was to develop effective topical antifungal formulation containing sertaconazole nitrate.

Background: Sertaconazole nitrate, topical antifungal was incorporated in solid-liquid lipid nanostructures and gelled further for topical application.

Objective: The objective of this investigation was to develop a topical formulation containing sertaconazole nitrate which was incorporated in the solid state of matrix to prolong the release in deep skin infection and hence reduce the application frequency.

Methods: The nanostructured lipid carriers of sertaconazole nitrate were developed by high speed homogenization followed by ultrasonication using Estosoft-GTS® (glyceryl tristearate) as a solid lipid, oleic acid as liquid lipid and Tween 80 as an emulsifier. Central composite design was used to optimize total lipid concentration and fraction of liquid lipid in the total lipid and its effect on entrapment efficiency and drug release was determined.

Results: The carrier particles had an average size of 366.3 nm; entrapment efficiency in between 50.66% to 87.36%; cumulative drug release up to 92.90% and zeta potential of 7.43 mV. Characterization by FTIR indicated no negative interaction between drug and excipients, XRD showed disappearance of crystalline peaks of the encapsulated drug while DSC revealed complete solubilization of the drug. About 99.6% of drug was estimated by HPLC method. The drug release from gel and cream was 25.04% and 72.97% respectively. The lipid and gel excipients did not interfere with antifungal activity of the drug.

Conclusion: The developed nanocarriers loaded gel were stable. It prolonged the drug release (for 24 hours) than marketed cream. It could be a promising concept for topical delivery of antifungal and anti-inflammatory materials.

Keywords:

Sertaconazole nitrate, nanostructured lipid carriers, central composite design, topical hydrogel, antifungal activity.

Affiliation:

Department of Pharmaceutics, MET’s Institute of Pharmacy, Nashik 422 003, affiliated to Savitribai Phule Pune University, Pune, Department of Pharmaceutics, MET’s Institute of Pharmacy, Nashik 422 003, affiliated to Savitribai Phule Pune University, Pune, Department of Analysis, MET’s Institute of Pharmacy, Nashik 422 003, affiliated to Savitribai Phule Pune University, Pune, Department of Analysis, MET’s Institute of Pharmacy, Nashik 422 003, affiliated to Savitribai Phule Pune University, Pune, Department of Pharmaceutics, MET’s Institute of Pharmacy, Nashik 422 003, affiliated to Savitribai Phule Pune University, Pune



Read Full-Text article