Rashmi Sharma and Rishabha Malviya* Pages 1 - 11 ( 11 )
Aim: The aim of study was to develop nanoparticles consisting etoricoxib with ethanolic extract of ginger rhizome (GE) and basil leaves (BE) and evaluated against human skin cancer cells (melanoma, SK-MEL-2). Nanoparticles were further incorporated into gel and ointment and characterized.
Material: Concentration of extract was varied while etoricoxib remains constant in nanoparticles. Nanoparticles were incorporated into gel and ointment, prepared by using tamarind seed polysaccharide and aloe vera gel, respectively.
Result: All the batches of nanoparticles were evaluated for particle size and were found from 924 nm (N1) to 1084 nm (N9). The loading efficiency of etoricoxib varied form 66.8±0.05 % (N4) to 85.1±0.04 % (N9), for GE 60.3±0.04 % (N4) to 72.1±0.05 % (N9), for BE 59.5±0.04 % (N8) to 80.5±0.03 % (N9). The consistency of ointment and gel was found smooth and no greediness observed. The pH of the nanoparticles incorporated ointment was observed 6.2±0.023 (O6) to 6.2±0.089 (O4), viscosity was found from 0.70±0.098 (O3) to 1.130±0.092 (O1) gm/cm3 , spreadability in the range of 58.3±0.062 (O2) to 66.2±0.098 (O5) %. The pH of nanoparticles incorporated gel was observed 6.2±0.019 (G2) to 6.2±0.098 (G7), viscosity was found from 0.847±0.030 (G7) to 1.130±0.065 gm/cm3 (G9), spreadability in the range of 62.5±0.045 % (G5) to 70.51±0.056 % (G4). In vitro cytotoxic studies showed that nanoparticles incorporated gel formulation (G1) was able to control cell grown (SK-MEL-2).
Conclusion: It can be concluded that etoricoxib and herbal components consisting formulations were able to control human skin cancer cells.
Nanoparticles, gel formulation, herbal adjuvant, skin cancer, etoricoxib
Department of Pharmacy, School of Medical and Allied Sciences, Galgotias University, Plot No. 02, Sector 17-A, Greater Noida, Gautam Buddha Nagar, Uttar Pradesh, Department of Pharmacy, School of Medical and Allied Sciences, Galgotias University, Plot No. 02, Sector 17-A, Greater Noida, Gautam Buddha Nagar, Uttar Pradesh