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Giemsa Staining of Some Nano-formulations on Neuroblastoma Cell Line

Author(s):

Kuldeep Nigam and Shweta Dang*   Pages 1 - 6 ( 6 )

Abstract:


Background: Nano-formulations of various drugs and natural compounds are becoming popular day by day. Number of cosmetic, medicinal and herbal products are formulated using nano-carriers. However, one of the concerns using these carriers is alteration in morphology of cell by using such particles or droplets.

Methods: In the present work, Giemsa staining was used to study the morphological changes on mammalian Neuro-2a cell line upon treatment with prepared capsaicin loaded nanoemulsion (Caps-NE), baclofen loaded poly(lactic-co-glycolic acid) nanoparticles (BcfPLGA-NPs) and lamotrigine loaded poly(lactic-co-glycolic acid) nanoparticles (Ltg-PLGA-NPs). Caps-NE, Bcf-PLGA-NPs and LtgPLGA-NPs along with their respective aqueous drug counterparts were examined for visible morphological changes at their corresponding reported maximum plasma concentration (Cmax) and 10xCmax values.

Results: Microscopic images showed that drug loaded nanoemulsion/nanoparticles showed comparable cell viability and exhibited lesser cytotoxicity than the available aqueous drug forms at both the concentrations.

Conclusion: The prepared formulations can used as potentially safer option as compared to their aqueous counterparts.

Keywords:

Capsaicin, Baclofen, Lamotrigine, nanoparticles, nanoemulsions, Giemsa Staining.

Affiliation:

Department of Biotechnology, Jaypee Institute of Information Technology, NOIDA, Department of Biotechnology,Jaypee Institute of Information Technology



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