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Giemsa Staining of Some Nano-formulations on Neuroblastoma Cell Line

[ Vol. 11 , Issue. 3 ]

Author(s):

Kuldeep Nigam and Shweta Dang*   Pages 357 - 362 ( 6 )

Abstract:


Background: Nano-formulations of various drugs and natural compounds are becoming popular day by day. A number of cosmetic, medicinal and herbal products are formulated using nano-carriers. However, one of the concerns using these carriers is the alteration in morphology of cell by using such particles or droplets.

Methods: In the present work, Giemsa staining was used to study the morphological changes on mammalian Neuro-2a cell line upon treatment with prepared capsaicin loaded nanoemulsion (Caps-NE), baclofen loaded poly(lactic-co-glycolic acid) nanoparticles (Bcf-PLGA-NPs) and lamotrigine loaded poly(lactic-co-glycolic acid) nanoparticles (Ltg-PLGA-NPs). Caps-NE, Bcf- PLGA-NPs and Ltg-PLGA-NPs, along with their respective aqueous drug counterparts, were examined for visible morphological changes at their corresponding reported maximum plasma concentration (Cmax) and 10xCmax values.

Results: Microscopic images showed that drug loaded nanoemulsion/nanoparticles showed comparable cell viability and exhibited lesser cytotoxicity than the available aqueous drug forms at both the concentrations.

Conclusion: The prepared formulations can be used as potentially safer option as compared to their aqueous counterparts.

Keywords:

Capsaicin, baclofen, lamotrigine, nanoparticles, nanoemulsions, giemsa staining.

Affiliation:

Department of Biotechnology, Jaypee Institute of Information Technology, Noida, Department of Biotechnology, Jaypee Institute of Information Technology, Noida



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