Kuldeep Nigam and Shweta Dang* Pages 357 - 362 ( 6 )
Background: Nano-formulations of various drugs and natural compounds are becoming popular day by day. A number of cosmetic, medicinal and herbal products are formulated using nano-carriers. However, one of the concerns using these carriers is the alteration in morphology of cell by using such particles or droplets.Methods: In the present work, Giemsa staining was used to study the morphological changes on mammalian Neuro-2a cell line upon treatment with prepared capsaicin loaded nanoemulsion (Caps-NE), baclofen loaded poly(lactic-co-glycolic acid) nanoparticles (Bcf-PLGA-NPs) and lamotrigine loaded poly(lactic-co-glycolic acid) nanoparticles (Ltg-PLGA-NPs). Caps-NE, Bcf- PLGA-NPs and Ltg-PLGA-NPs, along with their respective aqueous drug counterparts, were examined for visible morphological changes at their corresponding reported maximum plasma concentration (Cmax) and 10xCmax values. Results: Microscopic images showed that drug loaded nanoemulsion/nanoparticles showed comparable cell viability and exhibited lesser cytotoxicity than the available aqueous drug forms at both the concentrations. Conclusion: The prepared formulations can be used as potentially safer option as compared to their aqueous counterparts.
Capsaicin, baclofen, lamotrigine, nanoparticles, nanoemulsions, giemsa staining.
Department of Biotechnology, Jaypee Institute of Information Technology, Noida, Department of Biotechnology, Jaypee Institute of Information Technology, Noida