Anjali Pandya, Rajani Athawale*, Durga Puro and Geeta Bhagwat Pages 53 - 66 ( 14 )
Background: The research work involves the development of PLGA biodegradable microspheres loaded with dexamethasone for intraocular delivery.
Objective: The study aimed to design and evaluate long acting PLGA microspheres for ocular delivery of dexamethasone.
Methods: The present formulation involves the development of long-acting dexamethasone loaded microspheres composed of a biodegradable controlled release polymer, Poly(D,L-lactide-coglycolide) (PLGA), for the treatment of posterior segment eye disorders intravitreally. PLGA with a monomer ratio of 50:50 of lactic acid to glycolic acid was used to achieve a drug release for up to 45 days. Quality by Design approach was utilized for designing the experiments. Single emulsion solvent evaporation technique along with high-pressure homogenization was used to facilitate the formation of microspheres.
Results: Particle size evaluation, drug content and drug entrapment efficiency were determined for the microspheres. Particle size and morphology were observed using Field Emission Gun- Scanning Electron Microscopy (FEG-SEM) and microspheres were in the size range of 1-5 μm. Assessment of drug release was done using in vitro studies and trans retinal permeation was observed by ex vivo studies using goat retinal tissues.
Conclusion: Considering the dire need for prolonged therapeutic effect on diseases of the posterior eye, an intravitreal long-acting formulation was designed. The use of biodegradable polymer with biocompatible degradation products was a rational approach to achieve this aim. The outcome of the present research shows that developed microspheres would provide a long-acting drug profile and reduce the frequency of administration thereby improving patient compliance.
Dexamethasone, PLGA microspheres, long acting, intravitreal, sensory organs, receptors.
C.U. Shah College of Pharmacy, S.N.D.T. Women’s University, Mumbai 400 049, Maharashtra, Prin. K.M. Kundnani College of Pharmacy, Cuffe Parade, Mumbai 400 005, Maharashtra, C.U. Shah College of Pharmacy, S.N.D.T. Women’s University, Mumbai 400 049, Maharashtra, C.U. Shah College of Pharmacy, S.N.D.T. Women’s University, Mumbai 400 049, Maharashtra