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Study of the Fluorescence Based Applications of Water Soluble (N,P) Doped Carbon Dots Synthesized via Microwave Assisted Green Pyrolysis

Author(s):

Rajshree Singh, Shikha Singh, Sudipta Senapati , Kheyanath Mitra, Jaydeep Singh, Susanta K Sen Gupta , Nira Misra, Pralay Maiti and Biswajit Ray*   Pages 1 - 13 ( 13 )

Abstract:


Water soluble nitrogen and phosphorus doped carbon dots (CD) has been synthesized using citric acid, tris(2-aminoethyl)amine and orthophosphoric acid via one step microwave assisted pyrolysis method. The synthesized CD has been characterized using FTIR, UV-Vis, fluorescence spectroscopy and EDAX coupled with SEM techniques. SEM study has shown the formation of nanosized CD with average size of ~18 nm. Elemental analysis via EDAX has confirmed successful incorporation of nitrogen (30.8% wt) and phosphorus (5.7% wt) atoms in it. The steady state and 3D fluorescence spectroscopic studies have shown its efficient fluorescence emission with emission maxima in the region of ~450 nm. It has shown efficient “turn off” fluorescence behaviour towards transition metal ions like Fe2+ and Co2+ ions and toxic nitro/phenolic aromatic compounds like p-nitrophenol and picric acid. The efficient interaction of it with BSA has been revealed in terms of fluorescence quenching of BSA using steady state, synchronous and 3D fluorescence spectroscopy. It has shown very good in vitro biocompatibility and enhanced cell adhesion properties towards NIH 3T3 fibroblast cells. Moreover, fluorescence microscopy has shown significant uptake of CD by the tested cell line. So, it may also find usefulness in fluorescence based bio-imaging.

Keywords:

water soluble (N&P) doped carbon dots, fluorescence based sensing, cell imaging

Affiliation:

Department of Chemistry, Institute of Science, Banaras Hindu University, Varanasi – 221005, Department of Chemistry, Institute of Science, Banaras Hindu University, Varanasi – 221005, School of Material Science and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi – 221005, School of Material Science and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi – 221005, Department of Chemistry, Institute of Science, Banaras Hindu University, Varanasi – 221005, Department of Chemistry, Institute of Science, Banaras Hindu University, Varanasi – 221005, School of Biomedical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi – 221005, School of Material Science and Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi – 221005, Department of Chemistry, Institute of Science, Banaras Hindu University, Varanasi – 221005



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