Sabiha Zamani, Danish Idrees, Babita Jha and Anal K. Jha* Pages 806 - 816 ( 11 )
Background: At the present time, silver and gold nanoparticles are emerging as promising agents for cancer therapy. The anticancer activities of these nanoparticles have been evaluated against numerous human cancer cell lines. Still, few reports were existing against the breast cancer cell lines and most of these studies have mainly used chemically mediated nanoparticles.
Objective: This study reports the in vitro anticancer activity of bioinspired Nanoparticles like, silver and gold against MCF-7 cells (breast cancer cell line) as well as HEK293 cell (human embryonic kidney cell line) by MTT assay.
Methods: These metal nanoparticles, Ag-NPs and Au-NPs were biologically synthesized using Adiantum sp. aqueous leaves extract. The Adiantum sp. can reduce HAuCl4 solution to Au-NPs and AgNO3 to Ag-NPs within 30 mins. The formation of NPs was confirmed by characterization techniques such as UV-vis spectrophotometer, SEM and XRD studies. Additionally, the anticancer activity was analysed by cell viability (3(4,5-dimethly-thiazol-2-yl)- 2,5-diphenyl tetrazo-lium bromide assay).
Results: The synthesized nanoparticles from Adiantum sp. were characterized by UV-visible spectroscopy. The role of functional groups was analysed using a Fourier Transform Infrared (FTIR) spectrophotometer. The XRD pattern clearly exemplified that the nanoparticles formed in this present synthesis are crystalline in nature. Furthermore, in MTT assay study, both NPs have shown cytotoxicity at different concentrations ranging from 2.5 to 100 μg/ml. The data reveal that the NPs from Adiantum explicitly, inhibits the viability of cancerous cell only and are non-cytotoxic to HEK293 cells in the tested concentration range.
Conclusion: The results provide a preliminary guidance that Adiantum mediated silver and gold nanoparticles might be used to treat breast cancer; however, it necessitates clinical studies to ascertain their potential as anticancer agents.
Silver nanoparticles, gold nanoparticles, pteridophytes, Adiantum sp., MCF-7 cells, nanotechnology.
Aryabhatta Center of Nanoscience and Nanotechnology, Bihar, School of Life Sciences, Jawaharlal Nehru University, New Delhi-11067, Aryabhatta Center of Nanoscience and Nanotechnology, Bihar, Aryabhatta Center of Nanoscience and Nanotechnology, Bihar