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Sensitive Detection of Human Hemoglobin by MWCNTs-ionic Liquid: Anthraquinone Modified Electrode

[ Vol. 9 , Issue. 4 ]

Author(s):

Aghdas Banaei, Mostafa Shourian, Fariba Dashtestani and Khadijeh Eskandari*   Pages 479 - 485 ( 7 )

Abstract:


Introduction: Today, nano-composite gels based on multi-walled carbon nanotubes in room temperature ionic liquid (MWCNTs-IL-Gel) are an interesting subject.

Materials and Methods: The nano-composite gels showed convenient electrochemical properties against redox activities of electroactive biomolecules. Also, the evaluation of hemoglobin concentration is a critical point in the clinical sample. So, the kind of nano-composite gel which is composed of NH2 functionalized MWCNTs and [amim] Br IL and anthraquinone 2-carboxilic acid (AQ) was fabricated and applied in electrochemical detection of hemoglobin. Cyclic voltammograms of NH2.MWCNTs-IL-AQ modified electrode exhibited redox peak sat -0.5 V (vs. Ag/AgCl) in 0.1 M phosphate buffer solution ((pH 7.0).

Results: As the hemoglobin added into the measured solution, the redox current decreased.

Conclusion: It can be concluded that the increment in the concentration of hemoglobin caused the decrease in the redox currents in cyclic voltammetry. The NH2.MWCNTs-IL-AQ would detect the concentration of hemoglobin from 1.64 x10−8 to 4.89x10−7 M.

Keywords:

Anthraquinone 2-carboxilic acid, electron transferring, hemoglobin, ionic liquid, amine functionalized multi-walled carbon nanotubes.

Affiliation:

Research Physics Group, Research Institute of Applied Science, Academic Center of Education, Culture and Research (ACECR), Tehran, Department of Biology, University of Guilan, Rasht, Department of Biology, Electronic Branch, Islamic Azad University, Tehran, Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran

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