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Synthesis and Characterization of L-Lysin Coated Iron Oxide Nanoparticles as Appropriate Choices for Cell Immobilization and Magnetic Separation

[ Vol. 9 , Issue. 4 ]

Author(s):

Mohammad Javad Raee, Alireza Ebrahiminezhad*, Mohammad Bagher Ghoshoon, Ahmad Gholami and Younes Ghasemi*   Pages 462 - 466 ( 5 )

Abstract:


Introduction: Cell separation is one of the important steps of purification in downstream processes. Some separation techniques such as centrifugation and filtration are expensive and would affect cell viability. Magnetic separation can be a good alternative for laboratory and industrial cell separation processes.

Methods: For this purpose, L-lysine coated Iron Oxide Nanoparticles (IONs) were synthesized and used for magnetic separation of Escherichia coli as the most applied microbial cell in biotechnological processes.

Results: IONs have successfully decorated the bacterial cells and cells were completely separated by applying an external magnetic field.

Conclusion: This study showed that coating of E. coli cells with IONs could help to isolate cells from culture media without using expensive instruments.

Keywords:

Downstream process, Escherichia coli, magnetic separation, iron oxide nanoparticles, cell immobilization, cell separation.

Affiliation:

Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz

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